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rabbit polyclonal anti phospho glua2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit polyclonal anti phospho glua2
    Key reagents and resources used in the present study.
    Rabbit Polyclonal Anti Phospho Glua2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+phospho+glua2/Phospho-AMPA+Receptor+2+(GluA2)+(Tyr869%2FTyr873%2FTyr876)+Antibody/pmc08481232-7-0-5
    Average 94 stars, based on 37 article reviews
    rabbit polyclonal anti phospho glua2 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "SALM4 negatively regulates NMDA receptor function and fear memory consolidation"

    Article Title: SALM4 negatively regulates NMDA receptor function and fear memory consolidation

    Journal: Communications Biology

    doi: 10.1038/s42003-021-02656-3

    Key reagents and resources used in the present study.
    Figure Legend Snippet: Key reagents and resources used in the present study.

    Techniques Used: Transduction, Virus, Recombinant, Software

    Related Articles

    Blocking Assay:

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model
    Article Snippet: The extracted whole protein samples (50 μg) or membrane protein samples (10 μg) were resolved by 8% SDS-PAGE gel and electroblotted onto PVDF membranes (Immobilon-P, Millipore) using Mini-PROTEAN 3 Electrophoresis System and Mini Trans-Blot Electrophoretic Transfer System (Bio-Rad). .. After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti-β-actin (1:3000 dilution; Sigma-Aldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R&D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R&D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology). .. After washing in Tris-buffered saline–Tween 20, the membranes were incubated with IRDye 700CW donkey anti-mouse IgG and IRDye 800CW Donkey anti-goat or rabbit IgG (1:10,000; LI-COR Biosciences) for 1.5 h at room temperature, and then scanned using an Odyssey near- infrared imaging scanner (Gene Company).

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model
    Article Snippet: The extracted whole protein samples (50 g) or membrane protein samples (10 g) were resolved by 8% SDS-PAGE gel and electroblotted onto PVDF membranes (Immobilon-P, Millipore) using Mini-PROTEAN 3 Electrophoresis System and Mini Trans-Blot Electrophoretic Transfer System (Bio-Rad). .. After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti- -actin (1:3000 dilution; SigmaAldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R&D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R&D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology). .. After washing in Tris-buffered saline–Tween 20, the membranes were incubated with IRDye 700CW donkey anti-mouse IgG and IRDye 800CW Donkey anti-goat or rabbit IgG (1:10,000; LI-COR Biosciences) for 1.5 h at room temperature, and then scanned using an Odyssey nearinfrared imaging scanner (Gene Company).

    Incubation:

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model
    Article Snippet: The extracted whole protein samples (50 μg) or membrane protein samples (10 μg) were resolved by 8% SDS-PAGE gel and electroblotted onto PVDF membranes (Immobilon-P, Millipore) using Mini-PROTEAN 3 Electrophoresis System and Mini Trans-Blot Electrophoretic Transfer System (Bio-Rad). .. After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti-β-actin (1:3000 dilution; Sigma-Aldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R&D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R&D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology). .. After washing in Tris-buffered saline–Tween 20, the membranes were incubated with IRDye 700CW donkey anti-mouse IgG and IRDye 800CW Donkey anti-goat or rabbit IgG (1:10,000; LI-COR Biosciences) for 1.5 h at room temperature, and then scanned using an Odyssey near- infrared imaging scanner (Gene Company).

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model
    Article Snippet: The extracted whole protein samples (50 g) or membrane protein samples (10 g) were resolved by 8% SDS-PAGE gel and electroblotted onto PVDF membranes (Immobilon-P, Millipore) using Mini-PROTEAN 3 Electrophoresis System and Mini Trans-Blot Electrophoretic Transfer System (Bio-Rad). .. After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti- -actin (1:3000 dilution; SigmaAldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R&D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R&D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R&D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R&D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology). .. After washing in Tris-buffered saline–Tween 20, the membranes were incubated with IRDye 700CW donkey anti-mouse IgG and IRDye 800CW Donkey anti-goat or rabbit IgG (1:10,000; LI-COR Biosciences) for 1.5 h at room temperature, and then scanned using an Odyssey nearinfrared imaging scanner (Gene Company).



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    Image Search Results


    Key reagents and resources used in the present study.

    Journal: Communications Biology

    Article Title: SALM4 negatively regulates NMDA receptor function and fear memory consolidation

    doi: 10.1038/s42003-021-02656-3

    Figure Lengend Snippet: Key reagents and resources used in the present study.

    Article Snippet: Rabbit polyclonal anti-phospho-GluA2 (Tyr869/Tyr873/Tyr876) , CST , #3921 (1 : 500) , .

    Techniques: Transduction, Virus, Recombinant, Software